Mobile Nucleic Acid Detection Shelter Laboratory

Mobile Nucleic Acid Detection Shelter Laboratory

The DNA extraction room should be a micro-negative pressure room, and an exhaust system must be set up to prevent the aerosol that may be generated during nucleic acid extraction from invading personnel and contaminating other program functions; the PCR amplification room should be a micro-negative pressure room , An exhaust system must be installed to prevent a large amount of amplification products from spreading and contaminating other program functions;

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Air organization:


1. The original reagent mixing room should be a positive pressure room, and it should be operated in a micro-environment 100-level ultra-clean bench. It is necessary to prevent the contamination of this operation room between other program functions.


2. The DNA extraction room should be a slightly negative pressure room, and an exhaust system must be set up to prevent the aerosol that may be generated during nucleic acid extraction from damaging personnel and spreading pollution to other program functions;


3. The PCR amplification room should be a slightly negative pressure room, and an exhaust system must be installed to prevent a large number of amplification products from spreading and contaminating other program functions;


4. The detection room should be a slight negative pressure room, and an exhaust system must be set up to prevent the diffusion and pollution of amplified products during the electrophoretic sequencing process. At the same time, because the 3130 analyzer has a large heat dissipation capacity, it will increase the indoor temperature by 3-5 degrees;


5. If the reagent mixing room, DNA extraction room, PCR amplification room, and detection room are equipped with a buffer room, the function room can be positive pressure, and the negative pressure is set in the buffer room, and the airflow is from the slit of the double door Respectively flow into the buffer room, and use a reasonable airflow organization to avoid cross-contamination between various functions;


6. The high-efficiency air supply outlet is the top air supply, and the side bottom return/exhaust air (shaft, clamping channel, buffer room).



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